NaOCl was significantly more cytotoxic than EDTA. The combination EDTA/CHX had an additive to antagonistic effect.ĬHX was significantly more cytotoxic than NaOCl and EDTA.
#Calcusyn serial serial#
Based on CalcuSyn modelling, a mainly antagonistic effect was recorded with NaOCl/CHX and NaOCl/EDTA combinations. experiment for cell viability using single micelle delivery vehicle were performed with serial dilution of the stock solution knowing that the ratio curcumin and platinum inside the micelle is also 1:1. The difference between CHX and NaOCl was significant (P < 0.05) as well as between NaOCl and EDTA (P < 0.05). CHX was the most cytotoxic irrigant tested, followed by NaOCl, whilst EDTA was the least cytotoxic irrigant tested. The tested irrigants were cytotoxic in dose- and time-dependent manner.
#Calcusyn serial software#
Analysis of cell survival and interaction of the irrigants was performed using CalcuSyn dose effect analyzer software to calculate a combination index (CI). Dose-response curves were plotted, and 50% inhibitory doses (IC50 ) were subjected to statistical analysis (anova and post hoc comparison test P < 0.05). The effect on cell survival was estimated after 6 and 24 h of exposure by means of the sulforhodamine B assay, in reference to controls. Growth medium was Dulbecco's modified Eagle's medium supplemented with 10% foetal bovine serum and antibiotics and was used as control. Cells were exposed to sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), chlorhexidine (CHX) and their combinations (NaOCl/EDTA, NaOCl/CHX, EDTA/CHX) in serial dilutions. od of Chou and Talalay (1984) by using CalcuSyn software (Chou and Hayball 1996).
MRC5 cells were grown as monolayer cultures at 37 ☌ in an atmosphere containing 5% CO2 in air and 100% relative humidity. A serial of 2 samples of 25 females (n : 50) was used for each dose. The hypothesis tested was that the irrigants were less cytotoxic when applied in combination than independently. To evaluate the capacity of commonly used root canal irrigants to induce cytotoxic effects, when applied singly or in combination.
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